caa a22 4500 465822673 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170937 doi (NATIONALLICENCE)springer-10.1007/BF00170937 Effect of oxygenation conditions on submerged cultures of Phanerochaete chrysosporium [Elektronische Daten] [Carlos Dosoretz, Austin Chen, Hans Grethlein] Summary: The effect of the oxygen supply pattern on the onset and development of the lignolytic enzyme system of Phanerochaete chrysosporium was studied in submerged culture employing the serum bottle approach. Periodic or continuous flushing through the head phase, and continuous bubbling through the liquid phase with either oxygen (O2) or air were applied. The nature of the O2 supply had a crucial regulatory effect not only on the formation of lignin-degrading peroxidases but also on their decay and on the production of extracellular protease activity and polysaccharides. Continuous oxygenation or aeration increased the glucose consumption rate, extracellular protease activity and polysaccharides. Gassing with air, whether continuous or periodic, sustained Mn-peroxidase activity while ligninase was undetectable. Continuous O2 supply speeded up ligninase decay, displaying a sharper maximum, while a broader maximum and slower decay of ligninase activity were observed when supplying periodic O2. Cultures initially grown with free exposure to air displayed a higher but sharper ligninase activity maximum when shifted to continuous rather than periodic O2 supply. In general, the higher levels of either polysaccharides or protease activity corresponded to the lower levels and faster decay of ligninase and Mn-peroxidase activities. Springer-Verlag, 1990 Dosoretz Carlos Department of Chemical Engineering and Center for Microbial Ecology, Michigan State University, 48824, East Lansing, MI, USA aut Chen Austin Michigan Biotechnology Institute, 3900 Collins Road, P. O. Box 27609, 48909, Lansing, MI, USA aut Grethlein Hans Department of Chemical Engineering and Center for Microbial Ecology, Michigan State University, 48824, East Lansing, MI, USA aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 131-137 0175-7598 34:1<131 1990 34 253 https://doi.org/10.1007/BF00170937 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170937 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dosoretz Carlos Department of Chemical Engineering and Center for Microbial Ecology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 700 1- Chen Austin Michigan Biotechnology Institute, 3900 Collins Road, P. O. Box 27609, 48909, Lansing, MI, USA aut NATIONALLICENCE 700 1- Grethlein Hans Department of Chemical Engineering and Center for Microbial Ecology, Michigan State University, 48824, East Lansing, MI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 131-137 0175-7598 34:1<131 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer