caa a22 4500 465822738 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170924 doi (NATIONALLICENCE)springer-10.1007/BF00170924 Alkaline serine protease produced from citric acid by Bacillus alcalophilus subsp. halodurans KP 1239 [Elektronische Daten] [Yukio Takii, Naohiro Kuriyama, Yuzuru Suzuki] Summary: Maximum production of alkaline serine protease by Bacillus alcalophilus subsp. halodurans KP 1239 was achieved after 24 h cultivation, at an initial pH of 7.6, on a medium containing 1.0% sodium citrate, 0.3% yeast extract, and 0.3% KH2PO4. The enzyme was purified to crystalline form from culture broth. The enzyme was most active at 60° C and at pH 11.5. The molecular weight, isoelectric point and sedimentation coefficient in water at 20° C were estimated as 29 000, 8.8 and 3.3S, respectively. The N-terminal amino acid sequence was Ala-Gln-Ser-Val-Pro-Trp-Gly-Ile-Ser-Arg-Val-Gln-Ala-Pro-Ala-Ala-His-Asn-Arg-Gly-. The enzyme shared its antigenic determinants with B. alcalophilus ATCC 21522 serine protease, but not with the subtilisins Carlsberg and BPN′. Springer-Verlag, 1990 Takii Yukio Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut Kuriyama Naohiro Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut Suzuki Yuzuru Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 57-62 0175-7598 34:1<57 1990 34 253 https://doi.org/10.1007/BF00170924 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170924 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takii Yukio Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Kuriyama Naohiro Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Suzuki Yuzuru Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 57-62 0175-7598 34:1<57 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer