caa a22 4500 465822754 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170930 doi (NATIONALLICENCE)springer-10.1007/BF00170930 Control of acyl lipid desaturation in the yeast Rhodotorula gracilis via the use of the cyclopropenoid fatty acid, sterculate [Elektronische Daten] [Carole Rolph, Rod Moreton, John Harwood] Summary: The effect of the cyclopropene fatty acid, sterculic acid, on fatty acid metabolism in the oleaginous yeast Rhodotorula gracilis (CBS 3043) has been studied. Sterculate caused approximately 90% inhibition of [1-14C]sterate desaturation but only about 25% inhibition when [1-14C]acetate was used as precursor. Measurement of acyl-coenzyme A (CoA) pool labelling and the pattern of distribution of radioactivity within lipid classes suggested that the high rate of inhibition of stearate desaturation was due to sterculate reducing the formation of stearoyl-CoA. In agreement with previous suggestions for plant and animal systems, experiments with a post-mitochondrial (20 000 g × 30 min) supernatant suggested that sterculoyl-CoA was the active inhibitor. The action of sterculate on endogenous lipid composition was most marked in stationary-phase cells. In such cells an increase in stearate was seen especially in the triacylglycerol pools, while α-linolenate was increased predominantly in the phospholipid fractions. The increased flux of carbon to α-linolenate at the same time that stearate desaturation appeared to be inhibited by sterculate, indicated that that two separate pathways for desaturation, employing different substrates, may operate in R. gracilis. We suggest that sterculate inhibits the stearoyl-(acyl-)CoA-dependent pathway but has little effect when phospholipids are used as substrates for acyl chain desaturation. Springer-Verlag, 1990 Rolph Carole Department of Biochemistry, University of Wales, College of Cardiff, P. O. Box 903, CF1 1ST, Cardiff, UK aut Moreton Rod Cadbury Schweppes plc, The University, Whiteknights, RG6 2LA, Reading, UK aut Harwood John Department of Biochemistry, University of Wales, College of Cardiff, P. O. Box 903, CF1 1ST, Cardiff, UK aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 91-96 0175-7598 34:1<91 1990 34 253 https://doi.org/10.1007/BF00170930 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170930 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rolph Carole Department of Biochemistry, University of Wales, College of Cardiff, P. O. Box 903, CF1 1ST, Cardiff, UK aut NATIONALLICENCE 700 1- Moreton Rod Cadbury Schweppes plc, The University, Whiteknights, RG6 2LA, Reading, UK aut NATIONALLICENCE 700 1- Harwood John Department of Biochemistry, University of Wales, College of Cardiff, P. O. Box 903, CF1 1ST, Cardiff, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 91-96 0175-7598 34:1<91 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer