caa a22 4500 465822770 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170928 doi (NATIONALLICENCE)springer-10.1007/BF00170928 Carbohydrate metabolism during submerged production of ergot alkaloids [Elektronische Daten] [Vladimira Caberc-Porekar, Matjana Didek-Brumec, Helena Sočič] Summary: The mycelial sugar composition and changes in specific activities of phosphofructokinase (PFK) and glucose-6-phosphate dehydrogenase, the key enzymes of the glycolytic and pentose-phosphate pathway of glucose catabolism, were followed throughout submerged fermentation of a high-yielding Claviceps purpurea L17 strain. Experimental data indicate that the pentose-phosphate pathway in glucose breakdown prevails during the vegetative phase of fermentation, the share of the glycolytic pathway becoming more pronounced during alkaloid synthesis. Both enzymes exhibit hyperbolic saturation kinetics, which is not usual for the PFK of eukaryotes. Springer-Verlag, 1990 Caberc-Porekar Vladimira Boris Kidrič Institute of Chemistry, Hajdrihova 19, P.O. Box 30, YU-61000, Ljubljana, Yugoslavia aut Didek-Brumec Matjana LEK, Pharmaceutical and Chemical Works, Celovška 135, YU-61000, Ljubljana, Yugoslavia aut Sočič Helena Boris Kidrič Institute of Chemistry, Hajdrihova 19, P.O. Box 30, YU-61000, Ljubljana, Yugoslavia aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 83-86 0175-7598 34:1<83 1990 34 253 https://doi.org/10.1007/BF00170928 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170928 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Caberc-Porekar Vladimira Boris Kidrič Institute of Chemistry, Hajdrihova 19, P.O. Box 30, YU-61000, Ljubljana, Yugoslavia aut NATIONALLICENCE 700 1- Didek-Brumec Matjana LEK, Pharmaceutical and Chemical Works, Celovška 135, YU-61000, Ljubljana, Yugoslavia aut NATIONALLICENCE 700 1- Sočič Helena Boris Kidrič Institute of Chemistry, Hajdrihova 19, P.O. Box 30, YU-61000, Ljubljana, Yugoslavia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 83-86 0175-7598 34:1<83 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer