caa a22 4500 465822789 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170914 doi (NATIONALLICENCE)springer-10.1007/BF00170914 Production of citric acid from methanol by a fluoroacetate-resistant mutant of Candida sp. Y-1 [Elektronische Daten] [Yoshiki Tani, Yasuyoshi Sakai, Shin-Gen Chou] Summary: Fermentative production of citric acid from methanol by an isolated yeast, Candida sp. Y-1, was investigated using a medium containing fluoroacetate, a potential inhibitor of aconitase. Culture conditions were optimized, and the results showed that efficient production of citric acid required several factors; (1) the optimum concentration of fluoroacetate, (2) an addition of yeast extract with corn steep liquor, (3) a low nitrogen source concentration, and (4) strictly aerobic conditions. We then isolated a fluoroacetate-resistant mutant strain MA92 with threefold higher citric acid productivity than the wild strain. This mutant strain had lower aconitase activity than the wild strain and produced 4.6 g/l citric acid from methanol after 4 days of culture. Springer-Verlag, 1990 Tani Yoshiki Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut Sakai Yasuyoshi Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut Chou Shin-Gen Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 5-9 0175-7598 34:1<5 1990 34 253 https://doi.org/10.1007/BF00170914 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170914 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tani Yoshiki Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Sakai Yasuyoshi Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Chou Shin-Gen Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 5-9 0175-7598 34:1<5 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer