caa a22 4500 465822827 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170931 doi (NATIONALLICENCE)springer-10.1007/BF00170931 Peptide and amino acid transport in Streptococcus bovis [Elektronische Daten] [Kenneth Westlake, Roderick Mackie] Summary: In amino acid transport studies with Streptococcus bovis using 14C-labelled amino acids, it has been shown that between 87% and 95% of cell-associated radioactivity was located in the cytosol. In similar studies with unlabelled peptides, most test peptide associated with S. bovis was truly intracellular. Using sodium dodecyl sulphate-polyacrylamide gel electrophoresis, the proteolytic activity in S. bovis was found to be largely cell-associated and of the serine-protease type, but stimulated by dithiothreitol. A wide range of extracellular peptide hydrolysing activities was demonstrated against the pentapeptide Leu-Trp-Met-Arg-Phe, which was completely hydrolysed to eight products after 10 min incubation. Some of this pentapeptide was transported intact, indicating the existence of mechanisms for the transport of peptides up to 751 Da. In studies with Arg-Phe-Ala, only Phe (F) and Ala (A), and to a much lesser extent Phe-Ala (FA) were transported after extracellular hydrolysis to FA, Arg (R), F and A. In this case, amino acid transport was much more predominant than peptide transport. The extent and nature of peptide transport was affected by the addition of protease inhibitors. Springer-Verlag, 1990 Westlake Kenneth Rumen Biochemistry, Animal and Dairy Science Research Institute, Private Bag X2, 1675, Irene, Republic of South Africa aut Mackie Roderick Rumen Biochemistry, Animal and Dairy Science Research Institute, Private Bag X2, 1675, Irene, Republic of South Africa aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 97-102 0175-7598 34:1<97 1990 34 253 https://doi.org/10.1007/BF00170931 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170931 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Westlake Kenneth Rumen Biochemistry, Animal and Dairy Science Research Institute, Private Bag X2, 1675, Irene, Republic of South Africa aut NATIONALLICENCE 700 1- Mackie Roderick Rumen Biochemistry, Animal and Dairy Science Research Institute, Private Bag X2, 1675, Irene, Republic of South Africa aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 97-102 0175-7598 34:1<97 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer