caa a22 4500 465822835 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170923 doi (NATIONALLICENCE)springer-10.1007/BF00170923 Characterization of pullulan-hydrolysing enzyme from an alkalopsychrotrophic Micrococcus sp [Elektronische Daten] [Takuhei Kimura, Koki Horikoshi] Summary: A pullulan-hydrolysing enzyme of Micrococcus sp. 207 was purified to an electrophoretically homogenous state by chromatography on DEAE-Toyopearl, α-cyclodextrin-Sepharose and Asahipak GS-520P. The purified enzyme was free of α-amylase activity. The molecular weight of the enzyme as estimated by SDS-PAGE was 120,000 and the pI value as determined by isoelectric focusing was 4.9. The enzyme was most active at pH 8.0 and 50°C. The enzyme was activated by the addition of CaCl2, but its thermoresistance increased after removing free Ca2+ ions. The enzyme could hydrolyse the α-1,6-linkages of amylopectins, glycogens and pullulan and the K m value for pullulan was about 0.018%. Pullulan at concentrations above 0.012% inhibited the enzyme activity and the activity was competitively inhibited by cyclodextrins. Springer-Verlag, 1990 Kimura Takuhei The Riken Institute, Hirosawa, 351-01, Wako, Saitama, Japan aut Horikoshi Koki The Riken Institute, Hirosawa, 351-01, Wako, Saitama, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 52-56 0175-7598 34:1<52 1990 34 253 https://doi.org/10.1007/BF00170923 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170923 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimura Takuhei The Riken Institute, Hirosawa, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 700 1- Horikoshi Koki The Riken Institute, Hirosawa, 351-01, Wako, Saitama, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 52-56 0175-7598 34:1<52 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer