caa a22 4500 46582286X CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170927 doi (NATIONALLICENCE)springer-10.1007/BF00170927 High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1 [Elektronische Daten] [Dieter Riesenberg, Klaus Menzel, Volkmar Schulz, Klaus Schumann, Gerhard Veith, Georg Zuber, Wolfgang Knorre] Summary: A defined medium was developed which, by means of a specific fed-batch mode, allows growth of the recombinant Escherichia coli strain TG1 (pBB210) up to a cell density of 60 g dry weight/l. Apart from glucose and aqueous ammonia fed as carbon and nitrogen sources, it was unnecessary to supply other nutrients or O2-enriched air. Aqueous ammonia also served for pH control. The pO2 level was kept at 20% saturation via closed-loop controls operating the two output variables of stirrer speed and glucose feeding rate. This fed-batch method prevented significant accumulation of acetate and other metabolic by-products. The recombinant E. coli expressed interferon alpha 1 more efficiently at a lower specific growth rate (μPr ≈ 0.15 h−1) than at the maximum specific growth rate (μmax = 0.45 h−1). Therefore, fermentation in the batch phase at μmax was only allowed to continue up to a medium cell density. In the succeeding fed-batch phase, the specific growth rate was reduced to μPr by increasing the stirrer speed according to an empirically developed time scale. Springer-Verlag, 1990 Riesenberg Dieter Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Menzel Klaus Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Schulz Volkmar Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Schumann Klaus Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Veith Gerhard Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Zuber Georg Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Knorre Wolfgang Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 77-82 0175-7598 34:1<77 1990 34 253 https://doi.org/10.1007/BF00170927 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170927 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Riesenberg Dieter Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Menzel Klaus Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Schulz Volkmar Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Schumann Klaus Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Veith Gerhard Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Zuber Georg Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 700 1- Knorre Wolfgang Zentralinstitut für Mikrobiologie und experimentelle Therapie der AdW der DDR, Beutenbergstrasse 11, 6900, Jena, German Democratic Republic aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 77-82 0175-7598 34:1<77 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer