caa a22 4500 465822878 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170933 doi (NATIONALLICENCE)springer-10.1007/BF00170933 Coupled reductive and oxidative degradation of 4-chloro-2-nitrophenol by a co-immobilized mixed culture system [Elektronische Daten] [Jürgen Beunink, Hans-Jürgen Rehm] Summary: The restriction of oxygen transfer in Ca-alginate beads used for the immobilization of microbial cells was applied to a coupled reductive and oxidative microbial degradation of the xenobiotic 4-chloro-2-nitrophenol (CNP). The conversion of CNP by Enterobacter cloacae under anaerobic conditions led to the formation of 4-chloro-2-aminophenol (CAP, 81%) and 4-chloro-2-acetaminophenol (CAAP, 16%) after 50 h incubation. CAP, the main reduction product, was further degraded under aerobic conditions by Alcaligenes sp. TK-2, a hybrid strain isolated by conjugative in-vivo gene transfer. Whereas both degradation steps excluded one another in homogeneous systems with free cells, a coupled reductive and oxidative degradation of CNP was observed in one aerated reactor system after co-immobilization of both strains in Ca alginate. The diameter of the alginate beads used for immobilization was recognized as one main factor determining the properties of this mixed culture system. Springer-Verlag, 1990 Beunink Jürgen Institut für Mikrobiologie, Universität Münster, Corresstrasse 3, D-4400, Münster, Federal Republic of Germany aut Rehm Hans-Jürgen Institut für Mikrobiologie, Universität Münster, Corresstrasse 3, D-4400, Münster, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 108-115 0175-7598 34:1<108 1990 34 253 https://doi.org/10.1007/BF00170933 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170933 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beunink Jürgen Institut für Mikrobiologie, Universität Münster, Corresstrasse 3, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 700 1- Rehm Hans-Jürgen Institut für Mikrobiologie, Universität Münster, Corresstrasse 3, D-4400, Münster, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 108-115 0175-7598 34:1<108 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer