caa a22 4500 465822894 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170926 doi (NATIONALLICENCE)springer-10.1007/BF00170926 Molecular cloning and expression of a xylanase gene from Cellulomonas sp. into Escherichia coli [Elektronische Daten] [J. Bhalerao, A. Patki, M. Bhave, I. Khurana, D. Deobagkar] Summary: The xylanase gene of Cellulomonas sp. NCIM 2353 was cloned in pUC 18 and selected by growth on xylan as the sole carbon source. The functional clone harboured the recombinant plasmid with an insert of 1.42 kbp, as determined by restriction mapping and Southern hydridization. The clone secreted a xylanase of 45 000 mol. wt. as determined by Western blot analysis using specific antixylanase antibodies. The DNA insert carried the full structural gene along with its promoter and possibly regulatory sequences, since xylanase activity in the clone Cs11 was inducible by xylan. Springer-Verlag, 1990 Bhalerao J. Department of Zoology, University of Poona, 411007, Pune, India aut Patki A. Department of Zoology, University of Poona, 411007, Pune, India aut Bhave M. Department of Zoology, University of Poona, 411007, Pune, India aut Khurana I. Department of Zoology, University of Poona, 411007, Pune, India aut Deobagkar D. Department of Zoology, University of Poona, 411007, Pune, India aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 71-76 0175-7598 34:1<71 1990 34 253 https://doi.org/10.1007/BF00170926 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170926 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bhalerao J. Department of Zoology, University of Poona, 411007, Pune, India aut NATIONALLICENCE 700 1- Patki A. Department of Zoology, University of Poona, 411007, Pune, India aut NATIONALLICENCE 700 1- Bhave M. Department of Zoology, University of Poona, 411007, Pune, India aut NATIONALLICENCE 700 1- Khurana I. Department of Zoology, University of Poona, 411007, Pune, India aut NATIONALLICENCE 700 1- Deobagkar D. Department of Zoology, University of Poona, 411007, Pune, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 71-76 0175-7598 34:1<71 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer