caa a22 4500 465822908 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170925 doi (NATIONALLICENCE)springer-10.1007/BF00170925 Improved transformation frequency and heterologous promoter recognition in Aspergillus niger [Elektronische Daten] [Georg Mohr, Karl Esser] Summary: Wild-type strains of Aspergillus niger were transformed with integrative vectors. The number of stable transformants varied from approximately 20-30/μg up to 17,000/μg using acetamide and hygromycin B selection, respectively. The introduction of deletions of 5′ and 3′ non-coding regions of the acetamidase gene (amdS+) revealed that these sequences influenced the number of transformants. The molecular characterization of A. niger transformants revealed that several copies of the vectors were tandemly integrated into the nuclear DNA. These oligomers were stably inherited, even after 100 days of growth on non-selective medium. The expression of the vector-encoded genes was confirmed by evidence from the mRNAs and corresponding proteins encoded by the selectable marker genes. Springer-Verlag, 1990 Mohr Georg Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Postfach 102148, Bochum, Federal Republic of Germany aut Esser Karl Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Postfach 102148, Bochum, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 63-70 0175-7598 34:1<63 1990 34 253 https://doi.org/10.1007/BF00170925 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170925 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohr Georg Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Postfach 102148, Bochum, Federal Republic of Germany aut NATIONALLICENCE 700 1- Esser Karl Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Postfach 102148, Bochum, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 63-70 0175-7598 34:1<63 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer