caa a22 4500 465822924 CHVBK 20180323112140.0 cr unu---uuuuu 170327e19901001xx s 000 0 eng 10.1007/BF00170921 doi (NATIONALLICENCE)springer-10.1007/BF00170921 Aryl acylamidase from Rhodococcus erythropolis NCIB 12273 [Elektronische Daten] [Peter Vaughan, Geoffrey Hall, David Best] Summary: A Rhodococcus erythropolis strain was isolated from soil on the basis of its ability to use acetaminophen as the sole source of both carbon and energy for growth. When grown in a complex medium containing an anilide inducer compound, the bacterium exhibited aryl acylamidase (EC 3.5.1.13) activity. This activity was not subject to carbon or nitrogen repression by the growth medium constituents as the enzyme was present throughout the exponential growth phase. The anilide was converted to the corresponding aniline, which was not further degraded. The enzyme was partially purified by a variety of methods including a batch ion exchange procedure, column ion exchange chromatography and hydrophobic interaction chromatography. The enzyme had a maximum activity at around pH 8.0 and had a Km for acetaminophen of 0.11 mM. Electrochemical assays of aryl acylamidase activity are described. The enzyme is suitable for use as a reagent in the clinical diagnostic measurement of acetaminophen. Springer-Verlag, 1990 Vaughan Peter MediSense (UK) Inc., OX14 1DY, Abingdon, Oxfordshire, UK aut Hall Geoffrey Biotechnology Centre, Cranfield Institute of Technology, MK43 0AL, Cranfield, Bedford, UK aut Best David Biotechnology Centre, Cranfield Institute of Technology, MK43 0AL, Cranfield, Bedford, UK aut Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 42-46 0175-7598 34:1<42 1990 34 253 https://doi.org/10.1007/BF00170921 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170921 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vaughan Peter MediSense (UK) Inc., OX14 1DY, Abingdon, Oxfordshire, UK aut NATIONALLICENCE 700 1- Hall Geoffrey Biotechnology Centre, Cranfield Institute of Technology, MK43 0AL, Cranfield, Bedford, UK aut NATIONALLICENCE 700 1- Best David Biotechnology Centre, Cranfield Institute of Technology, MK43 0AL, Cranfield, Bedford, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/1(1990-10-01), 42-46 0175-7598 34:1<42 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer