caa a22 4500 465822940 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170061 doi (NATIONALLICENCE)springer-10.1007/BF00170061 The genetic stability of Penicillium chrysogenum transformants in a fermentor [Elektronische Daten] [D. Renno, G. Saunders, A. Bull, G. Holt] Summary: A number of transformants of Penicillium chrysogenum have been obtained with the plasmid vector p3SR2. Southern analysis showed that transformation had occurred by integration of vector sequences into the nuclear DNA of the fungus. A number of transformants were tested for stability of the transformed phenotype in agar medium and some were found to be unstable. Two transformants, shown to be stable in agar culture, were grown in 5-l batch fermentors for further stability tests. Over periods of up to 312 h in the fermentor both transformants were 100% stable with respect to the transformed phenotype. In addition Southern analysis of DNA extracted from the spent mycelium showed that no change had occurred in the position of the integrated vector sequences within the transformant nuclear DNA. Springer-Verlag, 1990 Renno D. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut Saunders G. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut Bull A. The Biological Laboratory, The University of Canterbury, CT2 7NJ, Kent, UK aut Holt G. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 364-367 0175-7598 34:3<364 1990 34 253 https://doi.org/10.1007/BF00170061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Renno D. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut NATIONALLICENCE 700 1- Saunders G. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut NATIONALLICENCE 700 1- Bull A. The Biological Laboratory, The University of Canterbury, CT2 7NJ, Kent, UK aut NATIONALLICENCE 700 1- Holt G. Polytechnic of Central London, School of Biotechnology, 115 New Cavendish Street, W1M 8JS, London, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 364-367 0175-7598 34:3<364 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer