caa a22 4500 465822959 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170049 doi (NATIONALLICENCE)springer-10.1007/BF00170049 Heterologous protein secretion from Aspergillus niger in phosphate-buffered batch culture [Elektronische Daten] [David Archer, Ian Roberts, Donald MacKenzie] Summary: Aspergillus niger was grown in batch culture containing various initial concentrations of sodium phosphate buffer (pH 6.5). A wild-type strain of A. niger and a transformed strain producing hen egg-white lysozyme were studied. The maximum cell yield was attained in medium not supplemented with phosphate. In those cultures acidification of the medium resulted in a minimum of pH 2.0 before reverting to near neutrality. Increasing the initial levels of phosphate buffer reduced the fall in pH but lowered cell yields. Secreted levels of lysozyme were maximal in the 50-100 mm range of added phosphate buffer although mycelial yields were reduced by one third of mycelial yields in medium unsupplemented with phosphate buffer. Springer-Verlag, 1990 Archer David Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut Roberts Ian Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut MacKenzie Donald Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 313-315 0175-7598 34:3<313 1990 34 253 https://doi.org/10.1007/BF00170049 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170049 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Archer David Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut NATIONALLICENCE 700 1- Roberts Ian Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut NATIONALLICENCE 700 1- MacKenzie Donald Agricultural and Food Research Council Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 313-315 0175-7598 34:3<313 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer