caa a22 4500 465823009 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170047 doi (NATIONALLICENCE)springer-10.1007/BF00170047 Production of \-mannanases by Trichoderma harzianum E58 [Elektronische Daten] [J. Torrie, D. Senior, J. Saddler] Summary: The extracellular mannanase and endoglucanase activities of Trichoderma harzianum E58 were followed during growth of the fungus on 1% (w/v) mannose, Avicel, locust bean gum, konjac powder or the water-soluble fraction from stream-treated white spruce (SWS). Peak galactomannanase activities of 0.60 IU/ml and 0.66 IU/ml were detected in culture filtrates after 6-8 days growth on locust bean gum and Avicel respectively. When SWS or konjac powder were used as substrates, lower but relatively constant levels of activity were detected between 2 and 11 days of growth. Growth of the fungus on mannan-rich locust bean gum resulted in the highest specific glucomannanase and galactomannanase values. Although growth on 1% mannose failed to induce any mannanase activity, when 0.5% galactomannan was added with mannose, mannanase activity was detected in the culture filtrate. This indicated that mannanase production was not repressed in the presence of mannose. Samples were taken from each culture at the time of maximum galactomannanase activity. A protein profile obtained by isoelectric focusing was followed by a zymogram overlay to detect bands exhibiting galactomannanase, glucomannanase and endoglucanase activities. Several bands showed mannanase and endoglucananase activity. One band at pI 6.55 revealed both gluco- and galactomannanase activity and was free of detectable cellulase activity. Springer-Verlag, 1990 Torrie J. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut Senior D. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut Saddler J. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 303-307 0175-7598 34:3<303 1990 34 253 https://doi.org/10.1007/BF00170047 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170047 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Torrie J. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Senior D. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut NATIONALLICENCE 700 1- Saddler J. Department of Biotechnology and Chemistry, Forintek Canada Corporation, 800 Montreal Rd, K1G 3Z5, Ottawa, Ontario, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 303-307 0175-7598 34:3<303 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer