caa a22 4500 465823041 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170052 doi (NATIONALLICENCE)springer-10.1007/BF00170052 Microbial conversion of o -acetyltoluidide into 4′-hydroxy- o -acetyltoluidide [Elektronische Daten] [Toru Nagasawa, Hideki Yoshioka, Hideaki Yamada] Summary: Approximately 600 strains of various bacteria and actinomycetes were tested for their ability to hydroxylate o-acetyltoluidide (AT) at the 4'-position. Nocardia asteroides IFO 3384 and N. globerula IFO 13510 were selected as the best strains to catalyse the hydroxylation of AT. The product was isolated from culture] broth and identified as 4'-hydroxy-o-acetyltoluidide (4'-HAT). When AT was added during the course of cultivation of N. asteroides IFO 3384 and N. globerula IFO 13510, final concentrations of 1.0 mg/ml (a 23% molar conversion yield) and 1.5 mg/ml 4'-HAT (a 34% molar conversion yield) were obtained, respectively, from AT after 7 days cultivation. On the other hand, when resting cells of N. globerula IFO 13510 were used, the concentration of 4'-HAT attained was 4.4 mg/ml, a 69% molar conversion yield, after 4 days reaction. Springer-Verlag, 1990 Nagasawa Toru Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwaki-cho, Sakyo-ku, 606, Kyoto, Japan aut Yoshioka Hideki Nippon Kayaku Co., Ltd., 31-12 Shimo 3-chome, Kita-ku, 115, Tokyo, Japan aut Yamada Hideaki Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwaki-cho, Sakyo-ku, 606, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 325-329 0175-7598 34:3<325 1990 34 253 https://doi.org/10.1007/BF00170052 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170052 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nagasawa Toru Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwaki-cho, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Yoshioka Hideki Nippon Kayaku Co., Ltd., 31-12 Shimo 3-chome, Kita-ku, 115, Tokyo, Japan aut NATIONALLICENCE 700 1- Yamada Hideaki Department of Agricultural Chemistry, Kyoto University, Kitashirakawa, Oiwaki-cho, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 325-329 0175-7598 34:3<325 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer