caa a22 4500 465823076 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170048 doi (NATIONALLICENCE)springer-10.1007/BF00170048 Production of extracellular pectinase enzymes by a mutant (Pol6) of Penicillium occitanis [Elektronische Daten] [Sanjay Jain, Henri Durand, Gerard Tiraby] Summary: Penicillium occitanis strain Pol6, a mutant developed for hyperproduction of cellulase and pectinase enzymes was used for the study of extracellular pectinase production when pectins from different sources (apple and citrus) and with varying degree of esterification (DE) were used as inducers. Highly esterified citrus pectins were found to be suitable substrates for polygalacturonase, pectinase and pectin methyl esterase production, while low esterified citrus pectin favoured pectin lyase (PL) production. Apple pectins induced other hydrolytic enzymes (e.g., β-1,3-glucanase, β-glucosidase, β-galactosidase), in addition to pectolytic enzymes. Moreover, the combination of high and low esterified citrus pectins induced the production of a complete pectinase complex. The extent of degradation of the substrate and the affinity for PL decreased with decreasing DE irrespective of the source. There was no evidence of PL activity in this strain. No significant effect of cations (Ca++, Mn++, Na+) on PL activity was observed. However, EDTA (100 mm) inhibited 50% of the activity, when tested on highly esterified (rapid set citrus) pectin. Springer-Verlag, 1990 Jain Sanjay Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse Cédex, France aut Durand Henri Laboratoire CAYLA, Avenue de Larrieu, 31094, Toulouse Cédex, France aut Tiraby Gerard Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse Cédex, France aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 308-312 0175-7598 34:3<308 1990 34 253 https://doi.org/10.1007/BF00170048 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170048 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jain Sanjay Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Durand Henri Laboratoire CAYLA, Avenue de Larrieu, 31094, Toulouse Cédex, France aut NATIONALLICENCE 700 1- Tiraby Gerard Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse Cédex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 308-312 0175-7598 34:3<308 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer