caa a22 4500 465823084 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170064 doi (NATIONALLICENCE)springer-10.1007/BF00170064 Production and composition of phenylpyrrole metabolites prodcued by Pseudomonas cepacia [Elektronische Daten] [James Roitman, Noreen Mahoney, Wojciech Janisiewicz] Summary: In shaken cultures, a strain of Pseudomonas cepacia isolated from apple leaves produced pyrrolnitrin and four other phenylpyrrole antibiotics. The concentrations of these metabolites were determined at intervals for 7 days in three different media at two initial pH levels. Optical density measurements revealed maximum cell concentrations after 24 h in nutrient broth, after 48 h in King's B medium, and after 96 h in minimum salts solution. The effects caused by initiating fermentations at pH 5.8 rather than 7.0 were in most cases not dramatic, although in some instances, especially in minimum salts broth, higher concentrations of metabolites were produced with the lower initial pH. Concentrations of the phenylpyrrole antibiotics were greatly affected by choice of culture medium and incubation time. Concentrations of the two nitrophenyl metabolites, pyrrolnitrin and 2-chloropyrrolnitrin, rose throughout the 7-day incubation and were more than 20 times greater in minimum salts medium than in either King's B medium or nutrient broth. The maximum concentrations of each of the three aminophenyl metabolites (dichloroamino, trichloroamino and monochloroamino) occurred in different media, the monochloro compound in nutrient broth, the dichloro compound in Kings B medium and the trichloro compound in minimum salts medium. The time dependence of the concentrations of the five metabolites supports the proposed biosynthesis of these pyrroles from tryptophan by successive chlorinations followed by oxidation of the amino group at the end of the pathway. Springer-Verlag, 1990 Roitman James ARS, United States Department of Agriculture, Western Regional Research Center, 94710, Albany, CA, USA aut Mahoney Noreen ARS, United States Department of Agriculture, Western Regional Research Center, 94710, Albany, CA, USA aut Janisiewicz Wojciech ARS, United States Department o f Agriculture, Appalachian Fruit Research Station, 25430, Kearneysville, WV, USA aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 381-386 0175-7598 34:3<381 1990 34 253 https://doi.org/10.1007/BF00170064 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170064 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Roitman James ARS, United States Department of Agriculture, Western Regional Research Center, 94710, Albany, CA, USA aut NATIONALLICENCE 700 1- Mahoney Noreen ARS, United States Department of Agriculture, Western Regional Research Center, 94710, Albany, CA, USA aut NATIONALLICENCE 700 1- Janisiewicz Wojciech ARS, United States Department o f Agriculture, Appalachian Fruit Research Station, 25430, Kearneysville, WV, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 381-386 0175-7598 34:3<381 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer