caa a22 4500 465823106 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170053 doi (NATIONALLICENCE)springer-10.1007/BF00170053 Long-term continuous production of optically active 2-(4-chlorophenoxy)propanoic acid by yeast lipase in an organic solvent system [Elektronische Daten] [Toshiaki Fukui, Takuo Kawamoto, Kenji Sonomoto, Atsuo Tanaka] Summary: Long-term continuous optical resolution of 2-(4-chlorophenoxy)propanoic acid was carried out by stereoselective esterification with Celite-adsorbed lipase OF 360 from Candida cylindracea using n-tetradecanol as the second substrate in organic solvent systems. The water content of the Celite-adsorbed lipase affected productivity, 1.0 μl water·mg lipase−1 being optimal for preparation of the adsorbed lipase. Water-saturated carbon tetrachloride-isooctane (8:2, v/v) was found to be an excellent organic solvent for the continuous operation. The particle size of Celite had no effect on productivity. Under optimized conditions, the (R)-enantiomer of the acid was continuously esterified with high stereoselectivity in a packed-bed column reactor for 34 days. Furthermore, it was found that treatment of the reactor with acetone made it possible to restore productivity and extend the period of continuous operation for further 29 days. Springer-Verlag, 1990 Fukui Toshiaki Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut Kawamoto Takuo Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut Sonomoto Kenji Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut Tanaka Atsuo Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 330-334 0175-7598 34:3<330 1990 34 253 https://doi.org/10.1007/BF00170053 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170053 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fukui Toshiaki Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Kawamoto Takuo Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Sonomoto Kenji Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Tanaka Atsuo Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, 606, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 330-334 0175-7598 34:3<330 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer