caa a22 4500 465823122 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170045 doi (NATIONALLICENCE)springer-10.1007/BF00170045 Kourteva Yordanka Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut Characterization of an extracellular glycoprotein inhibitor of trypsin (TI-23) produced by Streptomyces sp. 23 [Elektronische Daten] [Yordanka Kourteva] Summary: The production of an extracellular trypsin inhibitor, TI-23, was found to parallel the growth of Streptomyces sp. 23 at different cultivation temperatures, reaching a maximum level at late exponential phase. Although the different temperatures (18°, 28° and 37°C) did not greatly affect the growth of the microorganism, they proved to be an important factor for extracellular inhibitory activity. Maximum specific rates of both cell growth and production of the inhibitor were recorded during the cultivation of Streptomyces sp. 23 at 37°C. TI-23 proved to be a monomeric glycoprotein containing 17% carbohydrate and differing in amino acid composition from the known extracellular proteinase inhibitors of streptomycetes. The molecular mass of the inhibitor was estimated to be about 13 kDa and the isoelectric point 4.3. The inhibition spectrum of TI-23 included trypsin as well as some microbial alkaline proteinases. Springer-Verlag, 1990 Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 292-296 0175-7598 34:3<292 1990 34 253 https://doi.org/10.1007/BF00170045 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170045 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Kourteva Yordanka Institute of Microbiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 292-296 0175-7598 34:3<292 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer