caa a22 4500 465823149 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170063 doi (NATIONALLICENCE)springer-10.1007/BF00170063 Activity of glycolytic enzymes of Saccharomyces cerevisiae in the presence of acetic acid [Elektronische Daten] [M. Pampulha, M. Loureiro-Dias] Summary: The effect of acetic acid on transport of glucose and on the activity of glycolytic enzymes of Saccharomyces cerevisiae was investigated. Acetic acid did not affect glucose transport. The inhibitory effect of the acid on the enzymes was considered from the point of view of acidification of the cytoplasm (pH dependence of the activity) and of the direct effect of the presence of acetic acid. Enolase was the enzyme most severely affected according to these two criteria. Fermentation was monitored in vivo by 31P-NMR. When ATP was available, a rise in cytoplasmic pH was observed and fermentation proceeded with a lower level of sugar phosphate. This may indicate that control was exerted at one of the early phosphorylation steps. Springer-Verlag, 1990 Pampulha M. Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, 7781, Codex, Portugal aut Loureiro-Dias M. Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, 7781, Codex, Portugal aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 375-380 0175-7598 34:3<375 1990 34 253 https://doi.org/10.1007/BF00170063 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170063 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pampulha M. Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, 7781, Codex, Portugal aut NATIONALLICENCE 700 1- Loureiro-Dias M. Laboratory of Microbiology, Gulbenkian Institute of Science, Oeiras, 7781, Codex, Portugal aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 375-380 0175-7598 34:3<375 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer