caa a22 4500 465823157 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901201xx s 000 0 eng 10.1007/BF00170059 doi (NATIONALLICENCE)springer-10.1007/BF00170059 Properties of cellobiase from Aspergillus niger [Elektronische Daten] [Ajay Singh, A. Agrawal, A. Abidi, N. Darmwal] Summary: Cellobiase enzyme was partially purified from the culture filtrate of Aspergillus niger AS-101 and the general and kinetic properties of the enzyme were examined. The enzyme was unstable on storage. However, it was protected by the addition of BSA, glycerol or sodium azide. Addition of glycerol also protected the enzyme from denaturation due to freezing and thawing. Effect of thiol group reagents revealed the presence of — SH groups at the active site of the enzyme. Different modulators such as metal ions and macroionic compounds illustrated varying effects on the purified cellobiase. Springer-Verlag, 1990 Singh Ajay Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut Agrawal A. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut Abidi A. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut Darmwal N. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 356-358 0175-7598 34:3<356 1990 34 253 https://doi.org/10.1007/BF00170059 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170059 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Singh Ajay Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut NATIONALLICENCE 700 1- Agrawal A. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut NATIONALLICENCE 700 1- Abidi A. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut NATIONALLICENCE 700 1- Darmwal N. Department of Biochemistry, N. D. University of Agriculture and Technology, 224229, Faizabad, India aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/3(1990-12-01), 356-358 0175-7598 34:3<356 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer