caa a22 4500 465823262 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166781 doi (NATIONALLICENCE)springer-10.1007/BF00166781 Synthesis and secretion of hirudin by Streptomyces lividans [Elektronische Daten] [Eckhard Bender, Renate Vogel, Klaus-Peter Koller, Joachim Engels] Summary: To examine the secretory production of heterologous proteins by Streptomyces lividans, we fused the DNA encoding the signal peptide of the α-amylase inhibitor tendamistat, derived from S. tendae with a synthetic gene encoding the thrombin inhibitor hirudin. The analysis of secretion by immunoblots revealed an efficient translocation of hirudin through the membrane, with no detectable immunoreaction among the cellular proteins. The secreted hirudin was stable in the shaking culture for about 6 days. A comparison of the hirudin secreted by S. lividans and recombinant reference hirudin from yeast by immunoblots and thrombin inhibition assays shows that hirudin from Streptomyces has a lower specific activity, which may be due to a different aminoterminal sequence or to inexact processing of the precursor. Springer-Verlag, 1990 Bender Eckhard Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut Vogel Renate Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut Koller Klaus-Peter Hoechst AG, Frankfurt/Main 80, D-6230, Federal Republic of Germany aut Engels Joachim Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 203-207 0175-7598 34:2<203 1990 34 253 https://doi.org/10.1007/BF00166781 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166781 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bender Eckhard Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut NATIONALLICENCE 700 1- Vogel Renate Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut NATIONALLICENCE 700 1- Koller Klaus-Peter Hoechst AG, Frankfurt/Main 80, D-6230, Federal Republic of Germany aut NATIONALLICENCE 700 1- Engels Joachim Institut für Organische Chemie, Universität Frankfurt, Frankfurt/Main 50, D-6000, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 203-207 0175-7598 34:2<203 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer