caa a22 4500 465823297 CHVBK 20180323112141.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166785 doi (NATIONALLICENCE)springer-10.1007/BF00166785 Genetic analysis of benzoate metabolism in Aspergillus niger [Elektronische Daten] [J. Boschloo, A. Paffen, T. Koot, W. van den Tweel, R. van Gorcom, J. Cordewener, C. Bos] Summary: The benzoate metabolism of Aspergillus niger was studied as part of a design to clone the benzoate-4-hydroxylase gene of this fungus on the basis of complementation. Filtration enrichment techniques yielded mutants defective for different steps of benzoate degradation: bph (benzoate-4-hydroxylase), phh (4-hydroxybenzoate-3-hydroxylase) and prc (protocatechuate ring cleavage) mutants. In this way the degradation pathway for benzoate, involving the formation of 4-hydroxybenzoate and 3,4-dihydroxybenzoate has been confirmed. In addition a mutant sensitive to benzoate has been found. Complementation tests in somatic diploids showed that the bph mutants belonged to two complementation groups. The major group is probably defective in the structural gene (bphA). All phh mutants tested belonged to one complementation group. The prc mutants could be divided into several groups on the basis of their growth on different aromatic substrates and on the basis of the complementation test. The phh and both bph mutations are shown to be located on different chromosomes. Springer-Verlag, 1990 Boschloo J. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Paffen A. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Koot T. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut van den Tweel W. DSM Research, Section Bio-organic chemistry, P. O. Box 18, NL-6160 MD, Geleen, The Netherlands aut van Gorcom R. Medical Biological Laboratory TNO, P. O. Box 45, NL-2280 AA, Rijswijk, The Netherlands aut Cordewener J. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Bos C. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 225-228 0175-7598 34:2<225 1990 34 253 https://doi.org/10.1007/BF00166785 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166785 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boschloo J. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Paffen A. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Koot T. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- van den Tweel W. DSM Research, Section Bio-organic chemistry, P. O. Box 18, NL-6160 MD, Geleen, The Netherlands aut NATIONALLICENCE 700 1- van Gorcom R. Medical Biological Laboratory TNO, P. O. Box 45, NL-2280 AA, Rijswijk, The Netherlands aut NATIONALLICENCE 700 1- Cordewener J. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bos C. Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 225-228 0175-7598 34:2<225 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer