caa a22 4500 465823319 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166775 doi (NATIONALLICENCE)springer-10.1007/BF00166775 Butyric fermentation: metabolic behaviour and production performance of Clostridium tyrobutyricum in a continuous culture with cell recycle [Elektronische Daten] [D. Michel-Savin, R. Marchal, J. Vandecasteele] Summary: Two physiological characteristics of butyric fermentation, inhibition by the acids produced, butyrate and acetate, and dependence on the growth rate of the distribution of these acids, prompted a study of butyrate production in a continuous fermentation system with cell recycle by microfiltration. The influence of the main operating parameters, glucose input (feed concentration and dilution rate) and bleed dilution rate on production of acids and biomass was studied. The performance of the system greatly exceeded the results obtained in batch and simple continuous fermentations as a high productivity for butyrate (9.5 g l−1 h−1) was achieved whilst retaining a satisfactory concentration of butyrate (29.7 g l−1) and low acetate production (0.6 g l−1) at a cell biomass concentration of 35 g l−1. Cell growth rate was found to be a critical parameter for performance stability as oscillations in metabolic activity due to inhibition by acids were observed at bleed dilution rates below 0.016 h−1. Springer-Verlag, 1990 Michel-Savin D. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut Marchal R. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut Vandecasteele J. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 172-177 0175-7598 34:2<172 1990 34 253 https://doi.org/10.1007/BF00166775 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166775 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Michel-Savin D. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 700 1- Marchal R. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 700 1- Vandecasteele J. Division Biotechnologie et Environment, Institut Français du Pétrole, F-92 506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 172-177 0175-7598 34:2<172 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer