caa a22 4500 46582336X CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166779 doi (NATIONALLICENCE)springer-10.1007/BF00166779 Feedback regulation and the intracellular protein profile of Streptomyces griseus in a cycloheximide fermentation [Elektronische Daten] [Kevin Dykstra, Henry Wang] Summary: Two-dimensional gel electrophoresis (2-D PAGE) was used to study the intracellular protein profile of Streptomyces griseus in relation to cycloheximide (CH) biosynthesis. Four proteins (CR1-CR4) were found to be significantly and specifically repressed by addition of the antibiotic (1 g/l at 72 h) to a producing fermentation. Synthesis of these proteins was specific to the idiophase, concurrent with CH production. Initial addition of CH to the production medium resulted in slightly lower synthesis rates of two of the proteins (CR1 and CR2), while significantly delaying the onset of synthesis of the other two (CR3 and C4). Finally, neutral polymetric resin was added to the fermentation to alleviate feedback regulation of CH synthesis, giving roughly a twofold increase in the antibiotic production rate. Production of proteins CR3 and CR4 was increased approximately tenfold immediately following resin addition, but returned to the control rate of synthesis after 24 h. Springer-Verlag, 1990 Dykstra Kevin Department of Chemical of Engineering, University of Michigan, 48 109, Ann Arbor, MI, USA aut Wang Henry Department of Chemical of Engineering, University of Michigan, 48 109, Ann Arbor, MI, USA aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 191-197 0175-7598 34:2<191 1990 34 253 https://doi.org/10.1007/BF00166779 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166779 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dykstra Kevin Department of Chemical of Engineering, University of Michigan, 48 109, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Wang Henry Department of Chemical of Engineering, University of Michigan, 48 109, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 191-197 0175-7598 34:2<191 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer