caa a22 4500 465823378 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166782 doi (NATIONALLICENCE)springer-10.1007/BF00166782 Regulation of extracellular protease production in Streptomyces clavuligerus [Elektronische Daten] [Victoria Bascarán, Carlos Hardisson, Alfredo Braña] Summary: The production of extracellular protease by Streptomyces clavuligerus was strongly influenced by the nature of the nitrogen source. Production took place in batch cultures during growth at low or intermediate growth rates, but was delayed to the post-exponential phase in media supporting high growth rates. Protease formation could be initiated by a nutritional shift-down induced by casamino acids deprivation. Under both types of conditions maximal production was related to the growth rates of the cultures and was stimulated by low concentrations of casamino acids or yeast extract. Some purine compounds also influenced production in shift-down conditions. Ammonium interfered with protease formation whenever it was added to the medium. Some mutants with ltered nitrogen control of primary metabolism were also affected in the production of protease. A partial characterization of the activity indicated that it was due to a single metalloprotease with an apparent molecular mass of 41,700 Da. Springer-Verlag, 1990 Bascarán Victoria Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut Hardisson Carlos Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut Braña Alfredo Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 208-213 0175-7598 34:2<208 1990 34 253 https://doi.org/10.1007/BF00166782 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166782 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bascarán Victoria Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut NATIONALLICENCE 700 1- Hardisson Carlos Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut NATIONALLICENCE 700 1- Braña Alfredo Laboratorio de Microbiologia, Departamento de Biología Functional, Facultad de Medicina, Universidad de Oviedo, 33 006, Oviedo, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 208-213 0175-7598 34:2<208 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer