caa a22 4500 465823386 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166783 doi (NATIONALLICENCE)springer-10.1007/BF00166783 Overproduction of an acetylxylan esterase from the extreme thermophile " Caldocellum saccharolyticum ” in Escherichia coli [Elektronische Daten] [Ernst Lüthi, Nila Jasmat, Peter Bergquist] Summary: The xynC gene coding for an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum” was overexpressed in Escherichia coli strain RR28 by cloning the gene downstream from the lacZ promoter region of pUC18 (pNZ1447) or downstream from the temperature-inducible λp r p l promoters of pJLA602 (pNZ1600). The protein formed high molecular weight aggregates in induced cells of RR28/pNZ1600 but not in RR28/pNZ1447. The enzyme constituted up to 10% of the total cell protein and was located in the cytoplasmic fraction of RR28/pNZ1447. The acetyl esterase was most active at pH 6.0 and 70-75° C with a half-life of 64 h at 70° C and 30 h at 80° C, respectively. Springer-Verlag, 1990 Lüthi Ernst Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut Jasmat Nila Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut Bergquist Peter Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 214-219 0175-7598 34:2<214 1990 34 253 https://doi.org/10.1007/BF00166783 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166783 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lüthi Ernst Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut NATIONALLICENCE 700 1- Jasmat Nila Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut NATIONALLICENCE 700 1- Bergquist Peter Centre for Gene Technology, Department of Cellular and Molecular Biology, University of Auckland, Auckland, New Zealand aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 214-219 0175-7598 34:2<214 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer