caa a22 4500 465823394 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166776 doi (NATIONALLICENCE)springer-10.1007/BF00166776 Production of glucosyl-xylose using Cellvibrio gilvus cells and its properties [Elektronische Daten] [Motomitsu Kitaoka, Hajime Taniguchi, Takashi Sasaki] Summary: 4-O-\-d-Glucopyranosyl-d-xylose (GX) was synthesized from equimolar amounts of d-xylose and α-d-glucose-1-phosphate (G-1-P) using acetone-treated cells of Cellvibrio gilvus. It was found that ethanol treatment of acetone-treated cells selectively removed phosphoglucomutase activity, which competes with cellobiose phosphorylase for G-1-P in the synthetic reaction. The yield of synthesis was 60%, based on d-xylose used. GX was purified by charcoal column chromatography with a 32% yield based on d-xylose. Nuclear magnetic resonance and fast atm bombardment mass data of GX are presented. The possibility for this saccharide to be used as a new foodstuff is also discussed. Springer-Verlag, 1990 Kitaoka Motomitsu Nippon Petrochemicals Co., Ltd., 1-3-1, Uchisaiwaicho, Chiyoda-ku, 100, Tokyo, Japan aut Taniguchi Hajime National Food Research Institute, 2-1-2, Kannondai, Tsukuba, 305, Ibaraki, Japan aut Sasaki Takashi National Food Research Institute, 2-1-2, Kannondai, Tsukuba, 305, Ibaraki, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 178-182 0175-7598 34:2<178 1990 34 253 https://doi.org/10.1007/BF00166776 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166776 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kitaoka Motomitsu Nippon Petrochemicals Co., Ltd., 1-3-1, Uchisaiwaicho, Chiyoda-ku, 100, Tokyo, Japan aut NATIONALLICENCE 700 1- Taniguchi Hajime National Food Research Institute, 2-1-2, Kannondai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 700 1- Sasaki Takashi National Food Research Institute, 2-1-2, Kannondai, Tsukuba, 305, Ibaraki, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 178-182 0175-7598 34:2<178 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer