caa a22 4500 465823432 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166784 doi (NATIONALLICENCE)springer-10.1007/BF00166784 Characterization of phage isolates from a phage-carrying culture of Leuconostoc oenos 58N [Elektronische Daten] [Elke Arendt, Horst Neve, Walter Hammes] Summary: During large-scale cultivation of Leuconostoc oenos strain 58N, growth inhibition was detected and attributed to the presence of the virulent phage P581. To determine if this phage originated from a temperate phage, L. oenos 58N was exposed to mitomycin C, and this treatment led indeed to release of phages (P58II). Further examination of the lytic potential of phages P581 and P58II revealed that these two phages were able to lyse the same strains of L. oenos with the exception of the original host strain, which was only sensitive to P581. Results of DNA/DNA hybridization experiments failed to show homology between the DNA of phage P58II and the chromosomal DNA of L. oenos 58N. A phage-free culture of L. oenos 58N could be obtained after repeated subculture. These results indicate that the original L. oenos 58N was in a special type of phage-carrier state. Phages P58I and P58II were compared on the basis of morphology, lytic spectra, restriction enzyme analysis, DNA homology, genome size and protein structure and proved to be identical. It is assumed that P58I arose from the phage-carrier culture of L. oenos 58N and became virulent by some mutational event. Springer-Verlag, 1990 Arendt Elke Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstra\e 25, D-7000, Stuttgart 70, Federal Republic of Germany aut Neve Horst Institut für Mikrobiologie, Bundesanstalt für Milchforschung, Hermann-Weigmann-Stra\e 1, D-2300, Kiel 1, Federal Republic of Germany aut Hammes Walter Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstra\e 25, D-7000, Stuttgart 70, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 220-224 0175-7598 34:2<220 1990 34 253 https://doi.org/10.1007/BF00166784 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166784 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arendt Elke Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstra\e 25, D-7000, Stuttgart 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Neve Horst Institut für Mikrobiologie, Bundesanstalt für Milchforschung, Hermann-Weigmann-Stra\e 1, D-2300, Kiel 1, Federal Republic of Germany aut NATIONALLICENCE 700 1- Hammes Walter Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstra\e 25, D-7000, Stuttgart 70, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 220-224 0175-7598 34:2<220 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer