caa a22 4500 465823475 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166788 doi (NATIONALLICENCE)springer-10.1007/BF00166788 Comparative study of the enzymatic synthesis of l -valine by purified enzymes, crude extract, intact or permeabilized cells from Bacillus megaterium [Elektronische Daten] [A. Honorat-Pascal, F. Monot, D. Ballerini] Summary: A detailed study on the reductive amination of α-ketoisovalerate to l-valine by l-valine dehydrogenase using glucose dehydrogenase as an NADH regeneration enzyme was performed. The presence of both enzyme activities in Bacillus megaterium ATCC 39 118 permitted a direct and systematic comparison of the performances (initial l-valine production rate, productivity, molar conversion yield) of different types of conversion systems: purified enzymes or crude extract and whole cells, intact or permeabilized. A maximal l-valine productivity of 8 mmol·l−1 · h−1 was obtained using purified enzymes which constituted the most efficient system with a maximal rate of 0.87 μmol · ml−1 · min−1 and a molar conversion yield of 0.91. Permeabilized cells were also an attractive system because of their easy preparation and of the good performances attained. Springer-Verlag, 1990 Honorat-Pascal A. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut Monot F. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut Ballerini D. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 236-241 0175-7598 34:2<236 1990 34 253 https://doi.org/10.1007/BF00166788 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166788 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Honorat-Pascal A. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 700 1- Monot F. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 700 1- Ballerini D. Institut Français du Pétrole, 1-4 Avenue de Bois-Préau, BP 311, F-92506, Rueil-Malmaison Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 236-241 0175-7598 34:2<236 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer