caa a22 4500 465823505 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166791 doi (NATIONALLICENCE)springer-10.1007/BF00166791 Anaerobic degradation of chlorophenol by an enrichment culture [Elektronische Daten] [G. Dietrich, J. Winter] Summary: An anaerobic mixed culture from sewage sludge was enriched in a yeast extract and peptone-containing medium; it was able to degrade 2-cholorophenol completely to methane and CO2. Degradation rates of 2-chlorophenol of up to 0.18 g/l per day were observed in suspended cultures without biomass retention and of 0.375 g/l per day in cultures immobilized on Liapor clay beads. Attempts to isolate the dechlorinating organism failed. The mixed culture was reduced to three morphologically distinctive microorganisms using a medium with limited amounts of yeast extract and peptone and n-butyrate as a co-substrate. Under these conditions the phenol-degrading bacterium was lost and phenol accumulated in the medium. No growth and no dehalogenation of 2-chlorophenol was obtained when yeast extract and peptone were omitted completely. Besides serving as a source of supplementary components, yeast extract and peptone were apparently required as the main source of carbon, wereas reducing equivalents for reductive dehalogenation were obtained by oxidation of n-butyrate. A spirochaete-like organism was presumably the dechlorinating bacterium. The mixed culture lost its dehalogenation capability if this organism was lost. n-Butyrate could be replaced by n-valerate, hexanoate, heptanoate, octanoate, pelargonic acid, n-decanoic acid or palmitate as co-substrates for dehalogenation of either 2-chlorophenol, 2-bromophenol or complete dechlorination of 2,6-dichlorophenol, whereas from 2,4-dichlorophenol only the substituent in the ortho-position could be eliminated. Springer-Verlag, 1990 Dietrich G. Institut für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, D-8400, Resensburg, Federal Republic of Germany aut Winter J. Institut für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, D-8400, Resensburg, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 253-258 0175-7598 34:2<253 1990 34 253 https://doi.org/10.1007/BF00166791 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166791 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dietrich G. Institut für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, D-8400, Resensburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Winter J. Institut für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, D-8400, Resensburg, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 253-258 0175-7598 34:2<253 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer