caa a22 4500 465823513 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166793 doi (NATIONALLICENCE)springer-10.1007/BF00166793 Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium [Elektronische Daten] [Bernard Kurek, Etiiene Odier] Summary: The lignin mineralization rate in cultures of Phanerochaete chrysosporium increases with lignin peroxidase concentration up to 20 nkat ml−1. At higher concentrations the rate of lignin mineralization decreases with increasing lignin peroxidase concentration. The amount of mycelium is not a limiting factor for lignin mineralization at high exocellular lignin peroxidase in association with the mycelium as pellets and no free exocellular enzyme induce a lignin mineralization rate equivalent to cultures reconstituted with washed pellets supplemented with 15 nkat ml−1 of exogenous free enzyme. These results show that although lignin degradation by lignin peroxidase seems to be facilitated when lignin peroxidase is localised on the surface of the mycelium, free exocellular lignin peroxidase can also efficiently enhance mineralization of lignin by P. chrysosporium. Springer-Verlag, 1990 Kurek Bernard Laboratoire de Microbiologie, Centre de Biotechnologie Agro-Industrielle, Institut National de la Recherche Agronomique, F-78850, Thiverval-Grignon, France aut Odier Etiiene Laboratoire de Microbiologie, Centre de Biotechnologie Agro-Industrielle, Institut National de la Recherche Agronomique, F-78850, Thiverval-Grignon, France aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 264-269 0175-7598 34:2<264 1990 34 253 https://doi.org/10.1007/BF00166793 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166793 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurek Bernard Laboratoire de Microbiologie, Centre de Biotechnologie Agro-Industrielle, Institut National de la Recherche Agronomique, F-78850, Thiverval-Grignon, France aut NATIONALLICENCE 700 1- Odier Etiiene Laboratoire de Microbiologie, Centre de Biotechnologie Agro-Industrielle, Institut National de la Recherche Agronomique, F-78850, Thiverval-Grignon, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 264-269 0175-7598 34:2<264 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer