caa a22 4500 465823521 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19901101xx s 000 0 eng 10.1007/BF00166797 doi (NATIONALLICENCE)springer-10.1007/BF00166797 Identification of a sheath-associated protein involved in phosphate transport in Sphaerotilus natans [Elektronische Daten] [Bryan Kaphammer, Charles Kulpa Jr] Summary: Sphaerotilus natans was shown to have a fourfold lower K′ mof phosphate transport when grown in medium containing 0.1 mm phosphate, compared to cells grown in 10.0 mm phosphate. Analysis of sheath proteins from cells grown at these two phosphate levels revealed a protein of 53 kDa present in the sheath of cells grown at a phosphate concentration of 0.1 mm. This sheath-associated, phosphate-regulated protein, designated SapP, was gel purified and used to raise a polyclonal antibody. Enzyme-linked immunosorbent assay was used to localize this protein to the surface of the sheathed cells. Phosphate uptake assays done in the presence of the antibody also showed a rise in the K′ mof phosphate transport in cells grown in 0.1 mm phosphate, indicating that this protein is involved in high-affinity phosphate transport. Springer-Verlag, 1990 Kaphammer Bryan Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA aut Kulpa Jr Charles Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA aut Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 282-286 0175-7598 34:2<282 1990 34 253 https://doi.org/10.1007/BF00166797 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00166797 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kaphammer Bryan Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA aut NATIONALLICENCE 700 1- Kulpa Jr Charles Department of Biological Sciences, University of Notre Dame, 46556, Notre Dame, Indiana, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 34/2(1990-11-01), 282-286 0175-7598 34:2<282 1990 34 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer