caa a22 4500 465823556 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170581 doi (NATIONALLICENCE)springer-10.1007/BF00170581 Cellulose fermentation by continuous cultures of Ruminococcus albus and Methanobrevibacter smithii [Elektronische Daten] [Spyros Pavlostathis, Terry Miller, Meyer Wolin] Summary: The hydrolysis and fermentation of cellulose (Avicel) by continuous cultures of Ruminococcus albus strain 7 and Methanobrevibacter smithii strain PS were studied. Cellulose destruction ranged from ca. 22% to 71% for 0.25 to 2.27 days solids retention time, respectively. The cellulose hydrolysis rate constant (k) was 1.3 days−1. Concentrations of soluble reducing sugars were low, showing that cellulose hydrolysis was the rate-limiting step of cellulose fermentation. The estimated methane-based molar growth yield for M. smithii was 2.8 g mol−1. Its maximum specific growth rate was ca. 4 days−1. The dissolved H2 half-saturation constant (K s ) for methanogenesis was ca. 1 μM. The final products of the co-culture were primarily acetate, CH4 and CO2 and low levels of ethanol and H2. The co-culture produced more H2 (used for reduction of CO2 to CH4) and acetate than a monoculture of R. albus. These differences coulb be accounted for by the lower production of ethanol, confirming to the theory of interspecies H2 transfer. Springer-Verlag, 1990 Pavlostathis Spyros Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut Miller Terry Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut Wolin Meyer Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 109-116 0175-7598 33:1<109 1990 33 253 https://doi.org/10.1007/BF00170581 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170581 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pavlostathis Spyros Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut NATIONALLICENCE 700 1- Miller Terry Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut NATIONALLICENCE 700 1- Wolin Meyer Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201-0509, Albany, NY, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 109-116 0175-7598 33:1<109 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer