caa a22 4500 465823564 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170567 doi (NATIONALLICENCE)springer-10.1007/BF00170567 Continuous production of baculovirus in a cascade of insect-cell reactors [Elektronische Daten] [F. van Lier, E. van den End, C. de Gooijer, J. Vlak, J. Tramper] Summary: Insect cells (Spodoptera frugiperda) were cultured in a continuous stirred-tank reactor. The effluent was led to a cascade of another two reactors, each containing half the volume of the cell-growth reactor, where the cells were infected with Autographa californica nuclear polyhedrosis virus. For about 10 days production of 107 polyhedra (virus particles embedded in a protein capsule) per cm3 was achieved. This short production time compared to previous experiments involving an analogous system with a single infection vessel of equal volume to the cell-growth vessel is ascribed to the accelerated occurence of the so-called passage effect (a decrease of infectious virus with time). From the results of a computer model it was concluded that this passage effect was accelerated by the change in residence time distribution as compared to the earlier experiments. time distribution as compared to the earlier experiments. Springer-Verlag, 1990 van Lier F. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut van den End E. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut de Gooijer C. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut Vlak J. Department of Virology, Agricultural University, P. O. Box 8045/EM, NL-6700, Wageningen, The Netherlands aut Tramper J. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 43-47 0175-7598 33:1<43 1990 33 253 https://doi.org/10.1007/BF00170567 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170567 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Lier F. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut NATIONALLICENCE 700 1- van den End E. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut NATIONALLICENCE 700 1- de Gooijer C. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut NATIONALLICENCE 700 1- Vlak J. Department of Virology, Agricultural University, P. O. Box 8045/EM, NL-6700, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Tramper J. Department of Food Science, Food and Bioengineering Group, Agricultural University, P. O. Box 81 29, NL-6700 EV, Wageningen aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 43-47 0175-7598 33:1<43 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer