caa a22 4500 465823637 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170571 doi (NATIONALLICENCE)springer-10.1007/BF00170571 Physiological aspects of surface-immobilized Catharanthus roseus cells [Elektronische Daten] [Denis Rho, Charles Bédard, Jean Archambault] Summary: Growth and alkaloid production of surface-immobilized C. roseus cells were studied in a 2-1 bioreactor. Media designed to maximize cell growth or alkaloid production were employed. Nitrate and carbohydrate consumption rates as well as growth rates and biomass yields of immobilized cultures were equal or somewhat lower than for cell suspension cultures. Respiration rate (O2 consumption and CO2 production rates) of immobilized C. roseus cell cultures was obtained by on-line analysis of inlet and outlet gas composition using a mass spectrometer. Respiration rate increased during the growth phase and decreased once the nitrogen or the carbon source was depleted from the medium. The respiration rate of immobilized C. roseus cells resembled rates reported in the literature for suspension cultures. Springer-Verlag, 1990 Rho Denis Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut Bédard Charles Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut Archambault Jean Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 59-65 0175-7598 33:1<59 1990 33 253 https://doi.org/10.1007/BF00170571 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170571 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rho Denis Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut NATIONALLICENCE 700 1- Bédard Charles Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut NATIONALLICENCE 700 1- Archambault Jean Biiotechnology Research Institute, National Research Council Canada, 6100 Royalmount, H4P 2R2, Montréal, Quebéc, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 59-65 0175-7598 33:1<59 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer