caa a22 4500 465823645 CHVBK 20180323112142.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170561 doi (NATIONALLICENCE)springer-10.1007/BF00170561 Water relations of polyol accumulation by Zygosaccharomyces rouxii in continuous culture [Elektronische Daten] [Petrus van Zyl, Bernard Prior] Summary: Glycerol and arabitol were the main polyols accumulated by Zygosaccharomyces rouxii in continuous culture but the intracellular and extracellular concentrations of the polyols varied with the dilution rate and osmoticum used to adjust the water activity (aw) to 0.960. When the aw was adjusted with NaCl, glycerol was the main polyol accumulated intracellularly whereas glycerol and arabitol were accumulated when polyethylene glycol (PEG) 400 was used. The extracellular glycerol and arabitol concentrations at 0.960 aw (NaCl or PEG 400) were similar or decreased relative to cultures at 0.998 aw. Compared to steady-state cultivation at 0.998 aw, the yeast retained at 0.960 aw (NaCl or PEG 400) a greater proportion of the total glycerol intracellularly against an increased concentration ratio without significantly greater production of glycerol. Arabitol was only significant in osmoregulation when cultivated at 0.960 aw (PEG 400). The intracellular glycerol concentration was insufficient to balance the aw across the membrane, but an equilibrium could be achieved under certain conditions if arabitol was also osmotically active. Springer-Verlag, 1990 van Zyl Petrus Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, Republic of South Africa aut Prior Bernard Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, Republic of South Africa aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 12-17 0175-7598 33:1<12 1990 33 253 https://doi.org/10.1007/BF00170561 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170561 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van Zyl Petrus Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, Republic of South Africa aut NATIONALLICENCE 700 1- Prior Bernard Department of Microbiology and Biochemistry, University of the Orange Free State, P.O. Box 339, 9300, Bloemfontein, Republic of South Africa aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 12-17 0175-7598 33:1<12 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer