caa a22 4500 465823696 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170564 doi (NATIONALLICENCE)springer-10.1007/BF00170564 The biosynthesis of proteases with fibrinolytic activity in immobilized cultures of Penicillium chrysogenum H9 [Elektronische Daten] [S. El-Aassar, H. El-Badry, A. Abdel-Fattah] Summary: The yield of fibrinolytic and other proteolytic enzymes by Ca-alginate immobilized cells of Penicillium chrysogenum H9 was compared to that of free cells. The gel matrix protected the immobilized cells from lysis providing higher stability to the biocatalyst. The highest fibrinolytic activity/caseinase activity ratio was obtained in immobilized cell cultures. The composition of culture medium influenced enzyme production, showing different patterns in free and immobilized cultures. An alginate concentration of 3% and 10 ml alginate beads/50 ml medium were optimum for fibrinolytic enzyme biosynthesis. Semicontinuous production of the enzyme indicated the superiority of immobilized cells. The beads were affected by repeated exposure to phosphate ions after 12 cycles. Springer-Verlag, 1990 El-Aassar S. Botany Department, Faculty of Science, Moharam Bey, Alexandria, Egypt aut El-Badry H. Botany Department, Faculty of Science, Moharam Bey, Alexandria, Egypt aut Abdel-Fattah A. Laboratory of Microbial Chemistry, National Research Center, Dokki, Cairo, Egypt aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 26-30 0175-7598 33:1<26 1990 33 253 https://doi.org/10.1007/BF00170564 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170564 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- El-Aassar S. Botany Department, Faculty of Science, Moharam Bey, Alexandria, Egypt aut NATIONALLICENCE 700 1- El-Badry H. Botany Department, Faculty of Science, Moharam Bey, Alexandria, Egypt aut NATIONALLICENCE 700 1- Abdel-Fattah A. Laboratory of Microbial Chemistry, National Research Center, Dokki, Cairo, Egypt aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 26-30 0175-7598 33:1<26 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer