caa a22 4500 465823750 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900401xx s 000 0 eng 10.1007/BF00170566 doi (NATIONALLICENCE)springer-10.1007/BF00170566 Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum [Elektronische Daten] [Peter Facchini, Frank DiCosmo] Summary: Cultured Catharanthus roseus cells were immobilized using geometrically identical needled fibreglass mats prepared with a range of surface coatings. The phenyl (PS), polyglycol (PG), aldehyde (CHO), alkyl (CTMS), and silanol (AW) coatings, along with the untreated glass (HC) surface, produced surfaces with a range of surface tensions. The immobilization efficiency of the substratum, measured as the percentage of cells immobilized, increased with increasing substratum surface tension in the order PS < PG < CHO < CTMS < AW < HC. The dependence of immobilization efficiency on substratum surface tension can be described using a thermodynamic model of adhesion that considers the extent of plant cell adhesion to be a function of the surface tensions of the substratum, the suspending liquid, and the plant cells. In addition, this dependence also demonstrates the fundamental role of adhesion in the immobilization process involving a glass fibre matrix. However, cell entrapment processes are also implicated. The untreated glass fibre substratum (HC), which demonstrated the greatest immobilization efficiency, was used for further characterization of the immobilization strategy. Maximum inoculum biomass was determined to be approximately 1.9 g cells (fresh weight)/g substratum (dry weight) to achieve greater than 90% immobilization efficiency. The growth rate of immobilized cultures was slower than suspension cultures, probably due to mass transfer limitations. Production of the indole alkaloids, tryptamine, catharanthine, and ajmalicine, was also suppressed relative to suspension-cultured cells. These results are considered in relation to other immobilization strategies and their apparent effects on cellular processes. Springer-Verlag, 1990 Facchini Peter Centre for Plant Biotechnology, Department of Botany, University of Toronto, 25 Willcocks Sreet, M5S 3B2, Toronto, Ontario, Canada aut DiCosmo Frank Centre for Plant Biotechnology, Department of Botany, University of Toronto, 25 Willcocks Sreet, M5S 3B2, Toronto, Ontario, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 36-42 0175-7598 33:1<36 1990 33 253 https://doi.org/10.1007/BF00170566 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00170566 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Facchini Peter Centre for Plant Biotechnology, Department of Botany, University of Toronto, 25 Willcocks Sreet, M5S 3B2, Toronto, Ontario, Canada aut NATIONALLICENCE 700 1- DiCosmo Frank Centre for Plant Biotechnology, Department of Botany, University of Toronto, 25 Willcocks Sreet, M5S 3B2, Toronto, Ontario, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/1(1990-04-01), 36-42 0175-7598 33:1<36 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer