caa a22 4500 465823815 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604941 doi (NATIONALLICENCE)springer-10.1007/BF00604941 Induction of heat shock proteins during initiation of solvent formation in Clostridium acetobutylicum [Elektronische Daten] [Andreas Pich, Franz Narberhaus, Hubert Bahl] Summary: The response to stresses produced by changes in the fermentation conditions ofClostridium acetobutylicum in continuous culture was determined under acid- and solvent-producing conditions. Using a phosphate-limited chemostat it was found that specificheatshockproteins (hsp 73, hsp 72 [Dnak], hsp 67 [GroEL], hsp 17 and hsp 14) were synthesized at elevated levels during the shift from acid to solvent formation. The induction of these stress proteins was observed before acetone and butanol were detected in the medium and was therefore not a response to these solvents present in the medium. Simultaneously with the induction of hsps, changes in the synthesis rates of other cellular proteins were observed. Synthesis of proteins associated with the acid production phase decreased and of proteins correlated with the solvent production phase increased. Some hsps, including the DnaK- and GroEL-similar proteins, hsp 73 and hsp 21, were also induced by a change in the growth rate and/or the pH. The analysis of the general regulation of the heat shock response inC. acetobutylicum revealed that the induction of at least 15 hsps after a temperature up-shift was transient and that two temporal classes of hsps could be distinguished. The synthesis of one group of hsps reached a maximum after 6 min and another around 11 min after the temperature upshift and returned to steady-state levels 30 to 40 min after the shock. Springer-Verlag, 1990 Pich Andreas Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut Narberhaus Franz Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut Bahl Hubert Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 697-704 0175-7598 33:6<697 1990 33 253 https://doi.org/10.1007/BF00604941 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604941 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pich Andreas Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Narberhaus Franz Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bahl Hubert Institut für Mikrobiologie, Georg-August-Universität, Grisebachstrasse 8, D-3400, Göttingen, Federal Republic of Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 697-704 0175-7598 33:6<697 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer