caa a22 4500 465823904 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604939 doi (NATIONALLICENCE)springer-10.1007/BF00604939 Cellulolytic properties of an extremely thermophilic anaerobe [Elektronische Daten] [J. Hudson, Hugh Morgan, R. Daniel] Summary: An extremely thermophilic anaerobe was isolated from a New Zealand hot spring by incubating bacterial mat strands in a medium containing xylan. The Gramreaction-negative organism that was subsequently purified had a temperature optimum of 70° C and a pH optimum of 7.0. The isolate, designated strain H173, grew on a restricted range of carbon sources. In batch culture H173 could degrade Avicel completely when supplied at 5 or 10 g l−1. There was an initial growth phase, during which a cellulase complex was produced and carbohydrates fermented to form acetic and lactic acids, followed by a phase where cells were not metabolising but the cellulase complex actively converted cellulose to glucose. When co-cultured with strain Rt8.B1, an ethanologenic extreme thermophile, glucose was fermented to ethanol and acetate, and no reducing sugars accumulated in the medium. In pH controlled batch culture H173 produced an increased amount of lactate and acetate but there was again a phase when reducing sugars accumulated in the medium, and these were converted to ethanol by co-culture with Rt8.B1. Springer-Verlag, 1990 Hudson J. Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut Morgan Hugh Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut Daniel R. Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 687-691 0175-7598 33:6<687 1990 33 253 https://doi.org/10.1007/BF00604939 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604939 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hudson J. Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut NATIONALLICENCE 700 1- Morgan Hugh Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut NATIONALLICENCE 700 1- Daniel R. Microbial Biochemistry and Biotechnology Unit, Department of Biological Sciences, University of Waikato, Private Bag, Hamilton, New Zealand aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 687-691 0175-7598 33:6<687 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer