caa a22 4500 465823939 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604924 doi (NATIONALLICENCE)springer-10.1007/BF00604924 Measurement of oxygen concentration gradients in gel-immobilized recombinant Escherichia coli [Elektronische Daten] [Christine Hooijmans, Catherine Briasco, Jun Huang, Bert Geraats, Jean-Noël Barbotin, Daniel Thomas, Karel Luyben] Summary: In this study, an oxygen microsensor was used to measure oxygen concentration profiles in carrageenan gel particles containing growing, immobilizedEcherichia coli B (pTG201). Profiles, which were measured at intervals during continuous culture of gel slabs and beads, became increasingly steep with time. The oxygen penetration depth in the gel decreased with time, eventually reaching a steady state value of approximately 100 μm for both gel beads and slabs. A reaction-diffusion model employing zero-order cell growth kinetics was found to provide an excellent fit to the experimental concentration data. Growth rates estimated from profiles obtained during the first few hours of culture were 0.24h−1 (gel slabs) and 0.18 h−1 (beads), compared to a value of 0.30 h−1 measured in free-cell suspensions at 25° C. Springer-Verlag, 1990 Hooijmans Christine Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Briasco Catherine Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut Huang Jun Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut Geraats Bert Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Barbotin Jean-Noël Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut Thomas Daniel Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut Luyben Karel Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 611-618 0175-7598 33:6<611 1990 33 253 https://doi.org/10.1007/BF00604924 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604924 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hooijmans Christine Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Briasco Catherine Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut NATIONALLICENCE 700 1- Huang Jun Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut NATIONALLICENCE 700 1- Geraats Bert Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Barbotin Jean-Noël Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut NATIONALLICENCE 700 1- Thomas Daniel Laboratoire de Technologie Enzymatique, URA N0523 du Centre Nationale de Recherches Scientifiques, Université de Technologie de Compiégne, BP 649, F-60 206, Compiègne, France aut NATIONALLICENCE 700 1- Luyben Karel Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 611-618 0175-7598 33:6<611 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer