caa a22 4500 465823947 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604935 doi (NATIONALLICENCE)springer-10.1007/BF00604935 Intergeneric hybridization between Monascus anka and Aspergillus oryzae by protoplast fusion [Elektronische Daten] [Hohzoh Kiyohara, Toshiro Watanabe, Junko Imai, Noboru Takizawa, Takashi Hatta, Kazutaka Nagao, Akira Yamamoto] Summary: To breed industrially useful strains of a slow-growing, red-pigment-producing strain ofMonascus anka, protoplasts ofM. anka MAK1 (arg) andAspergillus oryzae AOK1 (met, thr) were fused. A mixture of protoplasts prepared from mycelia ofM. anka MAK1 treated with 2% Usukizyme and ofA. oryzae AOK1 treated with 2% Usukizyme and 0.2% NovoZym 234 was incubated with 30% (w/v) polyethylene glycol no. 6000. Heterokaryon fusants complementing the auxotrophies of both mutants were isolated on minimal medium, but segregated into red (MAK1) and white (AOK1) sectors after being cultured on a complete medium. After irradiation with UV light, the fusants gave stable heterozygous diploids that formed long white hyphae. These diploids, which had twice as much DNA in the nucleus as their parents, grew more rapidly than the parent strain YZT1, and produced ethanol earlier than the parents. Production of amylase, protease, and kojic acid by the fusants was intermediate in amount between that of the two parents. Springer-Verlag, 1990 Kiyohara Hohzoh Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut Watanabe Toshiro Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut Imai Junko Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut Takizawa Noboru Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut Hatta Takashi Central Institute for Research, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut Nagao Kazutaka Faculty of Engineering, Hiruzen Research Institute, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut Yamamoto Akira Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 671-676 0175-7598 33:6<671 1990 33 253 https://doi.org/10.1007/BF00604935 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604935 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiyohara Hohzoh Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Watanabe Toshiro Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut NATIONALLICENCE 700 1- Imai Junko Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut NATIONALLICENCE 700 1- Takizawa Noboru Biotechnology Laboratory, Department of Applied Chemistry, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Hatta Takashi Central Institute for Research, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Nagao Kazutaka Faculty of Engineering, Hiruzen Research Institute, Okayama University of Science, 1-1 Ridai-cho, 700, Okayama, Japan aut NATIONALLICENCE 700 1- Yamamoto Akira Yaegaki Zymotecnics Co., 681 Mukudani, Hiyashida-cho, 679-42, Himeji, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 671-676 0175-7598 33:6<671 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer