caa a22 4500 465823971 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604927 doi (NATIONALLICENCE)springer-10.1007/BF00604927 Properties and repeated use of a reversibly soluble-insoluble yeast lytic enzyme [Elektronische Daten] [Masayuki Taniguchi, Seiichi Tanahashi, Michihiro Fujii] Summary: A yeast lytic enzyme was covalently immobilized on an enteric coating polymer, Eudragit S, that is reversibly soluble and insoluble (S-IS) depending on the pH of the reaction medium. The yeast lytic enzyme immobilized on Eudragit S (Y-E) showed a sharp response of solubility to slight changes in pH without decrease in enzymatic activity. The specific activity per amount of enzyme protein of Y-E for dry yeast cells was about two-thirds that of the native enzyme. In both lysis reactions of dry and pressed baker's yeast cells, changing the pH of the reaction medium from 7.0 to 4.8 at an appropriate interval allows the insoluble Y-E and the reaction products (soluble protein for dry yeast cells and invertase and soluble protein for pressed baker's yeast cells) to be repeatedly separated. The reaction method using a reversible S-IS enzyme is a promising procedure for repeated use of the enzyme in a heterogeneous reaction system containing yeast cells as a substrate. Springer-Verlag, 1990 Taniguchi Masayuki Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut Tanahashi Seiichi Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut Fujii Michihiro Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 629-632 0175-7598 33:6<629 1990 33 253 https://doi.org/10.1007/BF00604927 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604927 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taniguchi Masayuki Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut NATIONALLICENCE 700 1- Tanahashi Seiichi Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut NATIONALLICENCE 700 1- Fujii Michihiro Department of Material and Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi 2, 950-21, Niigata, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 629-632 0175-7598 33:6<629 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer