caa a22 4500 46582398X CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604934 doi (NATIONALLICENCE)springer-10.1007/BF00604934 Optimized transformation by electroporation of Lactobacillus plantarum strains with plasmid vectors [Elektronische Daten] [Françoise Bringel, Jean-Claude Hubert] Summary: Whole cell transformation ofLactobacillus plantarum CCM 1904 by electroporation was optimized. Pulse duration and electric field strength were shown to be important parameters: the optimum conditions were 12.5 kV/cm, a time-constant of 10 ms for an exponential decay waveform and 6.7 kV/cm applied during 2.5 ms for a square waveform. Transformation efficiency was increased if cells were cultivated on medium containing sorbitol and harvested during their early exponential growth phase: 8 × 10−4 transformants/μg pGK12 DNA per viable cell were obtained, with a survival rate of 10%-30% Cryotreatment by several freeze-and-thaw cycles decreased transformant yields. Transformation efficiency with different plasmids was studied and plasmid pGK12 was found to transformL. plantarum the most efficiently. Transformation by electroporation ofL. plantarum is strain dependent. The best results were obtained withL. plantarum NCIB 7220, giving 5 × 106 transformants/gmg plasmid pGK12 DNA. Springer-Verlag, 1990 Bringel Françoise Laboratoire de Microbiologie, Université Louis-Pasteur, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique (CNRS), 4, rue Blaise-Pascal, F-67070, Strasbourg Cedex, France aut Hubert Jean-Claude Laboratoire de Microbiologie, Université Louis-Pasteur, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique (CNRS), 4, rue Blaise-Pascal, F-67070, Strasbourg Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 664-670 0175-7598 33:6<664 1990 33 253 https://doi.org/10.1007/BF00604934 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604934 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bringel Françoise Laboratoire de Microbiologie, Université Louis-Pasteur, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique (CNRS), 4, rue Blaise-Pascal, F-67070, Strasbourg Cedex, France aut NATIONALLICENCE 700 1- Hubert Jean-Claude Laboratoire de Microbiologie, Université Louis-Pasteur, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique (CNRS), 4, rue Blaise-Pascal, F-67070, Strasbourg Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 664-670 0175-7598 33:6<664 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer