caa a22 4500 465823998 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900901xx s 000 0 eng 10.1007/BF00604930 doi (NATIONALLICENCE)springer-10.1007/BF00604930 Beauveria bassiana submerged conidia production in a defined medium containing chitin, two hexosamines or glucose [Elektronische Daten] [Dwayne Hegedus, Michael Bidochka, George Khachatourians] Summary: Beauveria bassiana in liquid culture can produce blastospores and occasionally submerged conidia. For use as a bioinsecticide, conidia have definite advantages. Numerous studies have investigated conidia production in liquid cultures using synthetic and industrial grade media supplemented with glucose. We have studied growth, development and sporulation in microcultures using growth media containing chitin monomers. For the production of submerged conidia growth media containing N-acetyl-d-glucosamine (GlcNAc) proved to be better than yeast extract-peptone-glucose (YPG), glucose plus ammonium salts (Glc+NH4Cl) or N-acetyl-d-galactosamine (GalNAc). Sixty-one percent of the spores in the GlcNAc medium were submerged conidia with the remainder being blastospores. The concentration of submerged conidia reached 8.0 × 105/ ml after two days in GlcNAc medium as compared to 8.9 × 105/ml in YPG medium. Therefore, in terms of percentage of submerged conidia produced, GlcNAc medium generated more submerged conidia in spite of its lower cell yields. Growth in a medium containing chitin, a polymer of GlcNAc, resulted in 86.3% of the spores as submerged conidia exceeding 106/ml after 48 h. Growth under phosphate limitation resulted in an increased percentage of submerged conidia for all media tested. Electron microscopy and spore protein analysis by sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed that structural and compositional differences exist between the spore types. Springer-Verlag, 1990 Hegedus Dwayne Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut Bidochka Michael Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut Khachatourians George Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 641-647 0175-7598 33:6<641 1990 33 253 https://doi.org/10.1007/BF00604930 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00604930 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hegedus Dwayne Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut NATIONALLICENCE 700 1- Bidochka Michael Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut NATIONALLICENCE 700 1- Khachatourians George Bioinsecticide Research Laboratory, Department of Applied Microbiology and Food Science, University of Saskatchewan, S7N 0W0, Saskatoon, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/6(1990-09-01), 641-647 0175-7598 33:6<641 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer