caa a22 4500 465824021 CHVBK 20180323112143.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164520 doi (NATIONALLICENCE)springer-10.1007/BF00164520 Two-stage continuous fermentation of Clostridium acetobutylicum: effects of pH and dilution rate [Elektronische Daten] [C. Godin, J. Engasser] Summary: The kinetics of a two-stage continuous fermentation of Clostridium acetobutylicum have been studied. The pH and the dilution rate have been shown to be two essential factors for process optimization. An increase in pH or dilution rate in the first stage decreased solvent production in the second fermentor. To achieve optimal solvent production, the pH had to be maintained at 4.5 in the first stage and between 4.5 and 5.0 in the second stage. Dilution rates of 0.08 h−1 and 0.04 h−1,respectively, in the first and second fermentors allowed a high solvent concentration. When the pH was maintained at 4.5 in each stage and when the dilution rates were 0.08 h−1 and 0.04 h−1 in the first and second fermentors respectively, 21 g/l solvent concentration was achieved. A conversion yield of 0.36 g solvents/g glucose consumed was obtained with total consumption of glucose. Biomass was only produced in the first stage together with 40% of the solvents, indicating that solvent production had to be induced in the first fermentor. Springer-Verlag, 1990 Godin C. Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-ENSAIA, Institut National Polytechnique de Lorraine, 1, rue Grandville, F-54042, Cedex, France aut Engasser J. Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-ENSAIA, Institut National Polytechnique de Lorraine, 1, rue Grandville, F-54042, Cedex, France aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 269-273 0175-7598 33:3<269 1990 33 253 https://doi.org/10.1007/BF00164520 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164520 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Godin C. Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-ENSAIA, Institut National Polytechnique de Lorraine, 1, rue Grandville, F-54042, Cedex, France aut NATIONALLICENCE 700 1- Engasser J. Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-ENSAIA, Institut National Polytechnique de Lorraine, 1, rue Grandville, F-54042, Cedex, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 269-273 0175-7598 33:3<269 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer