caa a22 4500 465824072 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164526 doi (NATIONALLICENCE)springer-10.1007/BF00164526 Integration of heterologous genes into the chromosome of Saccharomyces cerevisiae using a delta sequence of yeast retrotransposon Ty [Elektronische Daten] [Akira Sakai, Yuki Shimizu, Fumio Hishinuma] Summary: Distribution of a delta (δ) sequence of the Ty element on a chromosome of the yeast Saccharomyces cerevisiae was analysed by pulsed-field gel electrophoresis. More than 100 copies of the δ sequence were non-randomly distributed on the chromosome. Using the δ sequence as a recombination site, mouse α-amylase and human β-endorphin genes were introduced into the chromosomal DNA. The integration occurred on a particular chromosome in each case and the copy number was estimated as three to five. It was also found that single- or multi-copy integration occured at a single or multiple sites on the particular chromosome. The integrants secreted α-amylase and β-endorphin by three-to fivefold compared with single-copy integrants. This type of integration was mitotically stable over a period of 50 generations under non-selective conditions. Springer-Verlag, 1990 Sakai Akira Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut Shimizu Yuki Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut Hishinuma Fumio Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 302-306 0175-7598 33:3<302 1990 33 253 https://doi.org/10.1007/BF00164526 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164526 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakai Akira Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut NATIONALLICENCE 700 1- Shimizu Yuki Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut NATIONALLICENCE 700 1- Hishinuma Fumio Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, 194, Machida, Tokyo, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 302-306 0175-7598 33:3<302 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer