caa a22 4500 465824080 CHVBK 20180323112144.0 cr unu---uuuuu 170327e19900601xx s 000 0 eng 10.1007/BF00164536 doi (NATIONALLICENCE)springer-10.1007/BF00164536 Characteristics and N -terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate [Elektronische Daten] [Ian Forrester, Anthony Grabski, Chittra Mishra, Brian Kelley, W. Strickland, Gary Leatham, Richard Burgess] Summary: Extracellular culture filtrates from ligninolytic cultures of the lignin-degrading basidiomycete Lentinula (syn. Lentinus) edodes (Berk.) Pegler contained one major peroxidase when grown on a commercial oak-wood substrate. The peroxidase was purified by polyethylenimine clarification, anion-exchange chromatography, and hydrophobic-interaction HPLC. The enzyme (MnP1) was a heme-iron protein with an apparent molecular weight of 44 600 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels and an isoelectric point of pH 3.2. The native enzyme had an absorption maximum at 407 nm, which shifted to 420 nm upon H2O2 addition. The pyridine-hemochrome-absorption spectrum indicated that one heme group was present per enzyme as protoporphyrin IX. N-terminal amino acid sequencing showed that MnP1 had higher sequence homology with manganese peroxidases than with lignin peroxidases reported from Phanerochaete chrysosporium. L. edodes MnP1 was capable of oxidizing lignin and lignin-model compounds in the presence of manganese and H2O2. Springer-Verlag, 1990 Forrester Ian Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut Grabski Anthony Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut Mishra Chittra Repligen-Sandoz Research Corporation, 02173, Lexington, MA, USA aut Kelley Brian Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut Strickland W. Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut Leatham Gary USDA Forest Service, Forest Products Laboratory, Institute for Microbial and Biochemical Technology, 53705-2398, Madison, WI, USA aut Burgess Richard Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 359-365 0175-7598 33:3<359 1990 33 253 https://doi.org/10.1007/BF00164536 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00164536 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Forrester Ian Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut NATIONALLICENCE 700 1- Grabski Anthony Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut NATIONALLICENCE 700 1- Mishra Chittra Repligen-Sandoz Research Corporation, 02173, Lexington, MA, USA aut NATIONALLICENCE 700 1- Kelley Brian Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut NATIONALLICENCE 700 1- Strickland W. Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut NATIONALLICENCE 700 1- Leatham Gary USDA Forest Service, Forest Products Laboratory, Institute for Microbial and Biochemical Technology, 53705-2398, Madison, WI, USA aut NATIONALLICENCE 700 1- Burgess Richard Protein Purification Facility, University of Wisconsin Biotechnology Center, 53705, Madison, WI, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer-Verlag 33/3(1990-06-01), 359-365 0175-7598 33:3<359 1990 33 253 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer